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TTLL13 Protein (Tubulin Tyrosine Ligase-Like Family, Member 13) (AA 1-815) (His tag)

Crystallography grade TTLL13 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3074875
$15,588.27
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
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  • Target See all TTLL13 products
    TTLL13 (Tubulin Tyrosine Ligase-Like Family, Member 13 (TTLL13))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-815
    Origin
    Human
    Source
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TTLL13 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MEPSTCRTME SEEDYVEEKE SEKCVKEGVT NPSNSSQQAL LKADYKALKN GVPSPIMATK IPKKVIAPVD TGDLEAGRRK RRRKRRSLAI NLTNCKYESV RRAAQMCGLK EVGEDEEWTL YWTDCAVSLE RVMDMKRFQK INHFPGMTEI CRKDLLARNL NRMYKLYPSE YNIFPRTWCL PADYGDFQSY GRQRKARTYI CKPDSGCQGR GIFITRNPRE IKPGEHMICQ QYISKPLLID GFKFDMRVYV LITSCDPLRI FTYEEGLARF ATTPYMEPSH NNLDNVCMHL TNYAINKHNE NFVRDGAVGS KRKLSTLNIW LQEHSYNPGE LWGDIEDIII KTIISAHSVL RHNYRTCFPQ YLNGGTCACF EILGFDILLD HKLKPWLLEV NHSPSFTTDS CLDQEVKDAL LCDAMTLVNL RGCDKRKVME EDKRRVKERL FQCYRQPRES RKEKTESSHV AMLDQERYED SHLGKYRRIY PGPDTEKYAR FFKHNGSLFQ ETAASKAREE CARQQLEEIR LKQEQQETSG TKRQKARDQN QGESAGEKSR PRAGLQSLST HLAYRNRNWE KELLPGQLDT MRPQEIVEEE ELERMKALLQ RETLIRSLGI VEQLTRLQHP GPQGQKKLHE SRDRLGSQEL KSMSLVLLVL LRGAATEQGA PHFLHPVLPH ESIPRILGAL PSMNAAIPHV PRYHLQPKNF NWTGEPAAIN SCSLSMKKAG RCYFSSARIR LTSQGQASRR LEAINRVLAG SVPPTLTPKQ GYFLQPERVA SDSWTECTLP SMVNSEHRAA KVPLCPASAP MLQRSRALLN INQFR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TTLL13P Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TTLL13 (Tubulin Tyrosine Ligase-Like Family, Member 13 (TTLL13))
    Alternative Name
    TTLL13P (TTLL13 Products)
    Synonyms
    1700111A04Rik, 4921538O11, BC024578, tubulin tyrosine ligase like 13, pseudogene, tubulin tyrosine ligase-like family, member 13, TTLL13P, Ttll13
    Background
    Polyglutamylase which preferentially modifies alpha-tubulin. Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step (By similarity). {ECO:0000250|UniProtKB:A4Q9F6}.
    Molecular Weight
    94.6 kDa Including tag.
    UniProt
    A6NNM8
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