FOXR1 Protein (AA 1-292) (Strep Tag)
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- Target See all FOXR1 Proteins
- FOXR1 (Forkhead Box R1 (FOXR1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-292
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Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This FOXR1 protein is labelled with Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Sequence
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MGNELFLAFT TSHLPLAEQK LARYKLRIVK PPKLPLEKKP NPDKDGPDYE PNLWMWVNPN IVYPPGKLEV SGRRKREDLT STLPSSQPPQ KEEDASCSEA AGVESLSQSS SKRSPPRKRF AFSPSTWELT EEEEAEDQED SSSMALPSPH KRAPLQSRRL RQASSQAGRL WSRPPLNYFH LIALALRNSS PCGLNVQQIY SFTRKHFPFF RTAPEGWKNT VRHNLCFRDS FEKVPVSMQG GASTRPRSCL WKLTEEGHRR FAEEARALAS TRLESIQQCM SQPDVMPFLF DL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. - Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- FOXR1 (Forkhead Box R1 (FOXR1))
- Alternative Name
- FOXR1 (FOXR1 Products)
- Synonyms
- foxn5 Protein, dlnb13 Protein, FOXN5 Protein, Foxn5 Protein, Gm1115 Protein, forkhead box R1 Protein, forkhead box R1 L homeolog Protein, Foxr1 Protein, FOXR1 Protein, foxr1 Protein, LOC100301626 Protein, foxr1.L Protein
- Background
- Forkhead box protein R1 (Forkhead box protein N5),FUNCTION: Transcription factor which acts as both an activator and a repressor (PubMed:34723967). Activates transcription of a number of genes including the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2 which are involved in protection against oxidative stress (PubMed:34723967). Required for normal brain development (By similarity). {ECO:0000250|UniProtKB:Q3UTB7, ECO:0000269|PubMed:34723967}.
- Molecular Weight
- 33.3 kDa
- UniProt
- Q6PIV2
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