Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PYROXD2 Protein (AA 1-581) (Strep Tag)

custom-made C10ORF33 Origin: Human Host: Cell-free protein synthesis (CFPS) Recombinant > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
Catalog No. ABIN3086816
  • Target See all PYROXD2 (C10ORF33) Proteins
    PYROXD2 (C10ORF33) (Chromosome 10 Open Reading Frame 33 (C10ORF33))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-581
    Origin
    • 2
    • 1
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    Cell-free protein synthesis (CFPS)
    Purification tag / Conjugate
    This PYROXD2 protein is labelled with Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Brand
    AliCE®
    Sequence
    MAASGRGLCK AVAASPFPAW RRDNTEARGG LKPEYDAVVI GAGHNGLVAA AYLQRLGVNT AVFERRHVIG GAAVTEEIIP GFKFSRASYL LSLLRPQIYT DLELKKHGLR LHLRNPYSFT PMLEEGAGSK VPRCLLLGTD MAENQKQIAQ FSQKDAQVFP KYEEFMHRLA LAIDPLLDAA PVDMAAFQHG SLLQRMRSLS TLKPLLKAGR ILGAQLPRYY EVLTAPITKV LDQWFESEPL KATLATDAVI GAMTSPHTPG SGYVLLHHVM GGLEGMQGAW GYVQGGMGAL SDAIASSATT HGASIFTEKT VAKVQVNSEG CVQGVVLEDG TEVRSKMVLS NTSPQITFLK LTPQEWLPEE FLERISQLDT RSPVTKINVA VDRLPSFLAA PNAPRGQPLP HHQCSIHLNC EDTLLLHQAF EDAMDGLPSH RPVIELCIPS SLDPTLAPPG CHVVSLFTQY MPYTLAGGKA WDEQERDAYA DRVFDCIEVY APGFKDSVVG RDILTPPDLE RIFGLPGGNI FHCAMSLDQL YFARPVPLHS GYRCPLQGLY LCGSGAHPGG GVMGAAGRNA AHVAFRDLKS M
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Grade
    custom-made
    Top Product
    Discover our top product C10ORF33 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    12 months
  • Target
    PYROXD2 (C10ORF33) (Chromosome 10 Open Reading Frame 33 (C10ORF33))
    Alternative Name
    PYROXD2 (C10ORF33 Products)
    Background
    Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2 (EC 1.-.-.-),FUNCTION: Probable oxidoreductase that may play a role as regulator of mitochondrial function. {ECO:0000269|PubMed:31170524}.
    Molecular Weight
    63.1 kDa
    UniProt
    Q8N2H3
You are here:
Support