WDR36 Protein (AA 1-951) (Strep Tag)

Catalog No. ABIN3096391

Product Details

Target: WDR36 (WD Repeat Domain 36 (WDR36))

Protein Type: Recombinant

Protein Characteristics: AA 1-951

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This WDR36 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Brand: AliCE®

Sequence: MCCTEGSLRK RDSQRAPEAV LCLQLWQRTV PLDTLKGLGT CFPSGPELRG AGIAAAMERA SERRTASALF AGFRALGLFS NDIPHVVRFS ALKRRFYVTT CVGKSFHTYD VQKLSLVAVS NSVPQDICCM AADGRLVFAA YGNVFSAFAR NKEIVHTFKG HKAEIHFLQP FGDHIISVDT DGILIIWHIY SEEEYLQLTF DKSVFKISAI LHPSTYLNKI LLGSEQGSLQ LWNVKSNKLL YTFPGWKVGV TALQQAPAVD VVAIGLMSGQ VIIHNIKFNE TLMKFRQDWG PITSISFRTD GHPVMAAGSP CGHIGLWDLE DKKLINQMRN AHSTAIAGLT FLHREPLLVT NGADNALRIW IFDGPTGEGR LLRFRMGHSA PLTNIRYYGQ NGQQILSASQ DGTLQSFSTV HEKFNKSLGH GLINKKRVKR KGLQNTMSVR LPPITKFAAE EARESDWDGI IACHQGKLSC STWNYQKSTI GAYFLKPKEL KKDDITATAV DITSCGNFAV IGLSSGTVDV YNMQSGIHRG SFGKDQAHKG SVRGVAVDGL NQLTVTTGSE GLLKFWNFKN KILIHSVSLS SSPNIMLLHR DSGILGLALD DFSISVLDIE TRKIVREFSG HQGQINDMAF SPDGRWLISA AMDCSIRTWD LPSGCLIDCF LLDSAPLNVS MSPTGDFLAT SHVDHLGIYL WSNISLYSVV SLRPLPADYV PSIVMLPGTC QTQDVEVSEE TVEPSDELIE YDSPEQLNEQ LVTLSLLPES RWKNLLNLDV IKKKNKPKEP PKVPKSAPFF IPTIPGLVPR YAAPEQNNDP QQSKVVNLGV LAQKSDFCLK LEEGLVNNKY DTALNLLKES GPSGIETELR SLSPDCGGSI EVMQSFLKMI GMMLDRKRDF ELAQAYLALF LKLHLKMLPS EPVLLEEITN LSSQVEENWT HLQSLFNQSM CILNYLKSAL L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: WDR36 (WD Repeat Domain 36 (WDR36))

Alternative Name: WDR36 (WDR36 Products)

Background: WD repeat-containing protein 36 (T-cell activation WD repeat-containing protein) (TA-WDRP),FUNCTION: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in the nucleolar processing of SSU 18S rRNA (PubMed:34516797, PubMed:21051332). Involved in T-cell activation and highly coregulated with IL2 (PubMed:15177553). {ECO:0000269|PubMed:15177553, ECO:0000269|PubMed:21051332, ECO:0000269|PubMed:34516797}.

Molecular Weight: 105.3 kDa

UniProt: Q8NI36

Pathways: Regulation of Cell Size