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SLC39A9 Protein (AA 1-307) (Strep Tag)

SLC39A9 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, WB, SDS
Catalog No. ABIN3107449
  • Target See all SLC39A9 Proteins
    SLC39A9 (Solute Carrier Family 39 (Zinc Transporter), Member 9 (SLC39A9))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-307
    Origin
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This SLC39A9 protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MDDFISISLL SLAMLVGCYV AGIIPLAVNF SEERLKLVTV LGAGLLCGTA LAVIVPEGVH ALYEDILEGK HHQASETHNV IASDKAAEKS VVHEHEHSHD HTQLHAYIGV SLVLGFVFML LVDQIGNSHV HSTDDPEAAR SSNSKITTTL GLVVHAAADG VALGAAASTS QTSVQLIVFV AIMLHKAPAA FGLVSFLMHA GLERNRIRKH LLVFALAAPV MSMVTYLGLS KSSKEALSEV NATGVAMLFS AGTFLYVATV HVLPEVGGIG HSHKPDATGG RGLSRLEVAA LVLGCLIPLI LSVGHQH
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product SLC39A9 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SLC39A9 (Solute Carrier Family 39 (Zinc Transporter), Member 9 (SLC39A9))
    Alternative Name
    SLC39A9 (SLC39A9 Products)
    Synonyms
    ZIP-9 Protein, ZIP9 Protein, 2010002A02Rik Protein, 2610511I23Rik Protein, 4833420E20Rik Protein, AW209151 Protein, slc39a9 Protein, MGC83699 Protein, MGC89410 Protein, wu:fc56g12 Protein, zgc:101628 Protein, DKFZp469D105 Protein, solute carrier family 39 member 9 Protein, solute carrier family 39, member 9 Protein, solute carrier family 39 (zinc transporter), member 9 Protein, solute carrier family 39 member 9 S homeolog Protein, solute carrier family 39 member 9 L homeolog Protein, SLC39A9 Protein, Slc39a9 Protein, slc39a9.S Protein, slc39a9.L Protein, slc39a9 Protein
    Background
    Zinc transporter ZIP9 (Solute carrier family 39 member 9) (Zrt- and Irt-like protein 9) (ZIP-9),FUNCTION: Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis (PubMed:25014355, PubMed:19420709, PubMed:28219737). Participates in the zinc ions efflux out of the secretory compartments (PubMed:19420709). Regulates intracellular zinc level, resulting in the enhancement of AKT1 and MAPK3/MAPK1 (Erk1/2) phosphorylation in response to the BCR activation (PubMed:23505453). Also functions as a membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1 (By similarity). This pathway contributes to CLDN1 and CLDN5 expression and tight junction formation between adjacent Sertoli cells (By similarity). Mediates androgen-induced vascular endothelial cell proliferation through activation of an inhibitory G protein leading to the AKT1 and MAPK3/MAPK1 (Erk1/2) activation which in turn modulate inhibition (phosphorylation) of GSK3B and CCND1 transcription (PubMed:34555425). Moreover, has dual functions as a membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through an inhibitory G protein (Gi) that mediates both MAP kinase and zinc signaling leading to the androgen-dependent apoptotic process (PubMed:25014355, PubMed:28219737). {ECO:0000250|UniProtKB:Q3KR82, ECO:0000250|UniProtKB:Q8BFU1, ECO:0000269|PubMed:19420709, ECO:0000269|PubMed:23505453, ECO:0000269|PubMed:25014355, ECO:0000269|PubMed:28219737, ECO:0000269|PubMed:34555425}.
    Molecular Weight
    32.3 kDa
    UniProt
    Q9NUM3
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