SLC25A25 Protein (AA 1-469) (Strep Tag)

Catalog No. ABIN3115651

Product Details

Target: SLC25A25 (Solute Carrier Family 25 (Mitochondrial Carrier, Phosphate Carrier), Member 25 (SLC25A25))

Protein Type: Recombinant

Protein Characteristics: AA 1-469

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC25A25 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MLCLCLYVPV IGEAQTEFQY FESKGLPAEL KSIFKLSVFI PSQEFSTYRQ WKQKIVQAGD KDLDGQLDFE EFVHYLQDHE KKLRLVFKSL DKKNDGRIDA QEIMQSLRDL GVKISEQQAE KILKSMDKNG TMTIDWNEWR DYHLLHPVEN IPEIILYWKH STIFDVGENL TVPDEFTVEE RQTGMWWRHL VAGGGAGAVS RTCTAPLDRL KVLMQVHASR SNNMGIVGGF TQMIREGGAR SLWRGNGINV LKIAPESAIK FMAYEQIKRL VGSDQETLRI HERLVAGSLA GAIAQSSIYP MEVLKTRMAL RKTGQYSGML DCARRILARE GVAAFYKGYV PNMLGIIPYA GIDLAVYETL KNAWLQHYAV NSADPGVFVL LACGTMSSTC GQLASYPLAL VRTRMQAQAS IEGAPEVTMS SLFKHILRTE GAFGLYRGLA PNFMKVIPAV SISYVVYENL KITLGVQSR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC25A25 (Solute Carrier Family 25 (Mitochondrial Carrier, Phosphate Carrier), Member 25 (SLC25A25))

Alternative Name: SLC25A25 (SLC25A25 Products)

Synonyms: MCSC Protein, PCSCL Protein, RP11-395P17.4 Protein, SCAMC-2 Protein, 1110030N17Rik Protein, mKIAA1896 Protein, Mcsc Protein, Pcscl Protein, mcsc Protein, pcscl Protein, scamc-2 Protein, scamc2 Protein, slc25a Protein, slc25a25 Protein, wu:fb13d12 Protein, wu:fd14e03 Protein, zgc:77454 Protein, SLC25A25 Protein, solute carrier family 25 member 25 Protein, solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25 Protein, solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 L homeolog Protein, solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25a Protein, solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 Protein, SLC25A25 Protein, Slc25a25 Protein, slc25a25.L Protein, slc25a25a Protein, slc25a25 Protein

Background: Mitochondrial adenyl nucleotide antiporter SLC25A25 (Mitochondrial ATP-Mg/Pi carrier protein 3) (Mitochondrial Ca(2+)-dependent solute carrier protein 3) (Short calcium-binding mitochondrial carrier protein 2) (SCaMC-2) (Solute carrier family 25 member 25),FUNCTION: Electroneutral antiporter that most probably mediates the transport of adenyl nucleotides through the inner mitochondrial membrane. Originally identified as an ATP-magnesium/inorganic phosphate antiporter, it could have a broader specificity for adenyl nucleotides. By regulating the mitochondrial matrix adenyl nucleotide pool could adapt to changing cellular energetic demands and indirectly regulate adenyl nucleotide-dependent metabolic pathways. {ECO:0000269|PubMed:15123600, ECO:0000269|PubMed:34346195}.

Molecular Weight: 52.7 kDa

UniProt: Q6KCM7