EFHB Protein (AA 1-853) (Strep Tag)

Catalog No. ABIN3125899

Product Details

Target: EFHB (EF-Hand Domain Family, Member B (EFHB))

Protein Type: Recombinant

Protein Characteristics: AA 1-853

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This EFHB protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MCSFVRVGSP KPLQTSASPL EMSSLRRTRA PEISELGLTP EQKDDIRDRV LRGSKSPTEL GFDLRLEQDR KWRERMGSSE AKSPPCHALG VGLERHTISG TPTEMGNLGL HKGSAFQGSK PLGVLPGRVG PENKGLPPRL RYGGTLHPPF STVHASPLAA ESRRRPLAWG SAWTDAVVEK QPVVGLELRK EPEKEPTCVV MNPYPEMPPK EVDIGLPQTQ ESDEAKNTEP LIGLVREPSE CPFAQQPEEK KEPGSTEPGV EPPGNIRPIY SGKFFDRVPC WPSAGKVKPV GYRVATCLTE KLPRLMTPPE AKKYFNFRYP PAGAERVFYG RANDPQIAPY LTHGLRSKIS IPMGSLINPQ PITTFQQKIK DKKESIYFSH QRAPLGKSHD QTPGLPKGMD VINTTLGTPT IRELSVRDTV NPSKSFEDVL KEGQEGHDLY TVSHNDYFAG EAKNRKYNPA SFHRFNLYGI PTPHFNDGRT MAKALHWLHE LQMERGAKIV SKRVDDFKEK FQHKLGKVLD PIAETMNVPP GHTFGSCLHP EEYGAGDLIH YRSPDEYLRG KDHQRAVVAA ARHHLKKFNH QNFDTLQVAF RHYDKKGDGV IDRAELHEAC VQANLHLDKM LLDHLFDYCD VDQDGLINYL EFANFLNWKD RIPLKEHEKR VVVKGKKPDC ENVTDTSMGE AEPSLLINPE DIVPKEPGSS EETLRTIQRP GDKVSHQYKT TSSEINAVVG AVPSMCHPIF GVPTIRSDIS APRIRRVSDM NNYGDEGNAY SLLHPSIFSQ KGVFERDFFK TRSKEEISDI LTNIGVKLSK EEFENVWNLA SKKHQRGEVC VETIRNVLDE LLHADLVKCK TAM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EFHB (EF-Hand Domain Family, Member B (EFHB))

Alternative Name: Efhb (EFHB Products)

Synonyms: 4921525D22Rik Protein, EF-hand domain family member B Protein, EF-hand domain family, member B Protein, EF hand domain family, member B Protein, EFHB Protein, efhb Protein, Efhb Protein

Background: EF-hand domain-containing family member B (Cilia- and flagella-associated protein 21),FUNCTION: Cytosolic sensor for calcium, modulates the interaction of STIM1 and ORAI1 upon store depletion and the activation of store-operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to nucleus (By similarity). Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (By similarity). {ECO:0000250|UniProtKB:F1MMV1, ECO:0000250|UniProtKB:Q8N7U6}.

Molecular Weight: 95.7 kDa

UniProt: Q8CDU5