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ARHGEF10L Protein (AA 1-1280) (His tag)

Crystallography grade ARHGEF10L Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3130849
$24,004.89
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Will be delivered in 70 Business Days
  • Target See all ARHGEF10L Proteins
    ARHGEF10L (rho Guanine Nucleotide Exchange Factor (GEF) 10-Like (ARHGEF10L))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1280
    Origin
    Mouse
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ARHGEF10L protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MASSNPPPQP AIGAPLAPSA PGPSPEVEED SGEAFEFDDS DEEEDTSSGL VVPGLAPERD TEPSLICFDT VPGSDLDPAA APPQTEAPTV VSNGDAVGAA ISGVRRSSWK RKSSRRIDRF TFPALEEDVI YDDVPCESPD AHQPGAERGL VYEDVHRAGA PRETEDLGWS SSEFESYSED SGEETKPEAE PTKHRGSFQP KLSPDLTRLK ERYVRTKRDI LALRVGGRDM QELKLKCDCK MTQLMKAAKS GTRDGLEKTR MAVMRKVSFL HRKDVLGDSE EEDMGLLEVG VTDIKPPAPE LGPMPDGLSP QQVVRRHILG SIVQSEGSYV ESLKRILQDY RNPLMEMEPK ALSARKCQVV FFRVKEILHC HSMFQIALSS RVAEWDSTEK IGDLFVASFS KSMVLDVYSD YVNNFTNAMS IIKKACLTKP AFLEFLKRRQ VCSTDRVTLY GLMVKPVQRF PQFILLLQDM LKNTPRGHPD RLSLQLALTE LETLAEKLNE QKRLADQVAE IQQLTKSVSD RSSLNKLLTS GQRQLLLCET LTETVYGDRG QLIKSKERRV FLLNDMLVCA NINFKPSNHR GQLEISSLVP LGPKYVVKWN TALPQVQVVE VGQDGGTYDK DNLLIQHAGA KKATAAGQAQ NKVYLGPPRL FQELQDLQKD LAVVEQITLL ISTLHGTYQN LNMTVAQDWC LALQRLMRVK EEEIHSANKC RLRLLLPGKP DKSGRPISFM VVFITPNPLS KISWVNRLHL AKIGLREENQ PGWLCPDEDK KSKAPFWCPI LACCVPAFSS RTLSLQLGGL VHSPVNSPLL GFSAVSTSLP QGYLWVGGGQ EGAGGQVEIF SLNRPSPRTV KSFPVAAPVL CIEYIPDPEE EAEGAEESRA ATDPSVTVHP TVCLGLQDGS ILLYGSVDTG TQCLATCKSP GPQPVLCLRH SPFYLLAGLQ DGTLAAYPRT SGDIPWDLES PPMCITVGPG PIRTLLSLED AAWASCGPRV TVLDAATLQT QQSFEAHQDE AVSVTHMVKA GSGVWMAFSS GSSIRLFHTE TLEHLQEINI ATRTTFLLPG QKHLCVTSLL ICQGLLWVGT DQGVIVLLPV PRLEGIPKIT GKGMVSLNGH CGPVAFLAVA MSILAPDILR SDQEEAEGPQ AEEDKPDGQA HETVPGPDSH TARELTRKKG ILLQYRLRST AHLPGPLLSV REPAPADGSA LEHSEEDGSI YEMADDPDVW VRSRPCARDA HRKEICSVAI ISGGQGYRHF GGAPGGLSGR AAPCSETDST LLIWQVPLAL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arhgef10l Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ARHGEF10L (rho Guanine Nucleotide Exchange Factor (GEF) 10-Like (ARHGEF10L))
    Alternative Name
    Arhgef10l (ARHGEF10L Products)
    Synonyms
    GrinchGEF Protein, 2810441C07Rik Protein, mKIAA1626 Protein, Rho guanine nucleotide exchange factor 10 like Protein, Rho guanine nucleotide exchange factor (GEF) 10-like Protein, ARHGEF10L Protein, Arhgef10l Protein
    Background
    Acts as guanine nucleotide exchange factor (GEF) for RHOA, RHOB and RHOC. {ECO:0000250}.
    Molecular Weight
    140.9 kDa Including tag.
    UniProt
    A2AWP8
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