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Arx Protein (AA 1-564) (His tag)

Crystallography grade ARX Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3131427
$16,803.00
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Will be delivered in 60 Business Days
  • Target See all Arx (ARX) Proteins
    Arx (ARX) (Aristaless Related Homeobox (ARX))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-564
    Origin
    Mouse
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Arx protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MSNQYQEEGC SERPECKSKS PTLLSSYCID SILGRRSPCK MRLLGAAQSL PAPLASRADQ EKAMQGSPKS SSAPFEAELH LPPKLRRLYG PGGGRLLQGA AAAAAAAAAA AAAATATGTA GPRGEVPPPP PPAARPGERQ DSAGAVAAAA AAAAWDTLKI SQAPQVSISR SKSYRENGAP FVPPPPALDE LSGPGGVAHP EERLSAASGP GSAPAAGGGT GAEDDEEELL EDEEDEEEEE ELLEDDDEEL LEDDARALLK EPRRCSVATT GTVAAAAAAA AAAVATEGGE LSPKEELLLH PEDAEGKDGE DSVCLSAGSD SEEGLLKRKQ RRYRTTFTSY QLEELERAFQ KTHYPDVFTR EELAMRLDLT EARVQVWFQN RRAKWRKREK AGAQTHPPGL PFPGPLSATH PLSPYLDASP FPPHHPALDS AWTAAAAAAA AAFPSLPPPP GSASLPPSGA PLGLSTFLGA AVFRHPAFIS PAFGRLFSTM APLTSASTAA ALLRQPTPAV EGAVASGALA DPATAAADRR ASSIAALRLK AKEHAAQLTQ LNILPGTSTG KEVC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arx Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Arx (ARX) (Aristaless Related Homeobox (ARX))
    Alternative Name
    Arx (ARX Products)
    Synonyms
    CT121 Protein, EIEE1 Protein, ISSX Protein, MRX29 Protein, MRX32 Protein, MRX33 Protein, MRX36 Protein, MRX38 Protein, MRX43 Protein, MRX54 Protein, MRX76 Protein, MRX87 Protein, MRXS1 Protein, PRTS Protein, phox2a Protein, RGD1562672 Protein, arx Protein, arx-a Protein, arx-b Protein, arx2 Protein, ct121 Protein, issx Protein, mrx29 Protein, mrx32 Protein, mrx33 Protein, mrx36 Protein, mrx38 Protein, mrx43 Protein, mrx54 Protein, mrx76 Protein, mrx87 Protein, mrxs1 Protein, prt5 Protein, xARX Protein, aristaless related homeobox Protein, aristaless related homeobox a Protein, aristaless related homeobox L homeolog Protein, Arx Protein, ARX Protein, arxa Protein, arx.L Protein
    Background
    Transcription factor required for normal brain development. May be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate (By similarity). {ECO:0000250}.
    Molecular Weight
    59.4 kDa Including tag.
    UniProt
    O35085
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