Family with Sequence Similarity 178, Member A (FAM178A) (AA 1-1278) protein (His tag)

Details for Product No. ABIN3135765
Protein Name
  • C10orf6
  • 3632432H19
  • 6030443O07Rik
  • AU014917
  • FAM178A
  • SMC5-SMC6 complex localization factor 2
  • SLF2
  • Slf2
Protein Characteristics
AA 1-1278
1
Origin
Mouse (Murine)
1
Source
Insect Cells
1
Protein Type
Recombinant
Purification tag / Conjugate
This FAM178A protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Sequence MTRRCMPARP GFPSSPAPGS SPPRCHLRPG STAPAAAGKR TESPGDRKQS IIDFFKPAAK QDKHMLDSPQ KSNIKYRGNG LSITGTEQFE RKLSSPKKLK PKRMSSEESP ILEAFMKGGK EHHKDRGVHE SRRPCMSLSK YLPKGAGIYA PSSYRLPKEI KAQKKKHQSP ERRKSLFIHE SNREKNDRDR GKNSEDSRKQ ATATEGDIFK HSSRSISSRS SLSRHHPGES TLGARFQLSL ASYWREREQK KLRKEQMEQR INSENSFSEA SNLSLKSSGV GKNCKPRHEH SKHTEAVPGK SNLSTLENGH LSRKRSSSDS WELSGSKQNK FSDKRTEELC GLRPEKHKRT YHTKSKRVLS REAPRHIPSE RKVYQTHCTE DSWWCSALGR HSQGAGKTVS RGMSIASTLR LYLGRVISQL WKMDISQEKD HLQTRGNFQA LNRINSPTKE QRNSVDSDLK STKEPIIPKA RESFLEKRPD TSHQREKFIR HIALKTPGGV LRLEDIAKEP EDETDRSSAD SAPSNAGHHS SRNSDQVHSA STKETKIQKP HLPLPQEKST IKRASNLQKN KPAGSVTSKE TKLPLLSHVP SAVSSRVPLN AKNCTLPVPK KDKERSSSKE RSGHSTESSK HKEHRAKTIK AVSNESSGKN SGGSLHSEYA PPTASPPAAL EVVPSVPSPA APSDKESSGN SNAGSNALKR KFRGDFDSDE ESLGYTLESD EEEETLKSLE EIMALNFSRT PTTSGKPPAV SKGLRSQSSD MKEYAQSGTY TNTLERLVKE MEDTQRLDEL QKKLQEDIRQ GRGIKSPLRT GDQDSTDDGD GLLEEHREFL KKFSVTVDAI PDHHPGEEIF NFLNSGKIFN QYTLDLRDSG FIGESAVEKL ILKSGKTDQI FLTTQGFLTT AYHYVQCPVP VLKWLFRMMS VHTDCIVSVQ ILSTLMEITI RNDTFSDSPV WPWIPSLSDI AAVFFNMGVG FGSLFPLETL QPDFNEENLI SETQKTLGGK ESEDSPYSPV FSALPETNIL NVVKFLGLCT SIHPEGYQDG ELMLLILMLF KMSLEKELKQ IPLVDFQSLL INLMKNIRDW NTKVHELCLG INELSSHPHN LLWLVQLVPN WTSRGRQLRQ CLSLVMMSKL LDEKHEDIPN ANNLQISVLH RYLVQMKPSD LLKKMVLKKR AEQPNETIDD SLHLELEKQA YYLTYILLHL VGEVSCSHSL SSGQRKHFVL LCGALEKHVK CDIREDARLF YRTKVKDLVA RIHGKWQEII QNCRPTQGQL HDFWVPDS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Slf2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Protein Name
Background Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions. {ECO:0000250|UniProtKB:Q8IX21}.
Molecular Weight 144.9 kDa Including tag.
UniProt Q6P9P0
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
Image no. 1 for Family with Sequence Similarity 178, Member A (FAM178A) (AA 1-1278) protein (His tag) (ABIN3135765) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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