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SUZ12 Protein (AA 1-741) (Strep Tag)

SUZ12 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Catalog No. ABIN3136018
  • Target See all SUZ12 Proteins
    SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-741
    Origin
    Mouse
    Source
    • 1
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This SUZ12 protein is labelled with Strep Tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA
    Sequence
    MAPQKHGGGG GGGSGPSAGS GGGGFGGSAA AVAAAASGGK SGGGGCGGGG SYSASSSSAA AAAAAAGAAV LPVKKPKMEH VQADHELFLQ AFEKPTQIYR FLRTRNLIAP IFLHRTLTYM SHRNSRTSIK RKTFKVDDML SKVEKMKGEQ ESHSLSAHLQ LTFTGFFHKN DKPSQNSENE QNSVTLEVLL VKVCHKKRKD VSCPIRQVPT GKKQVPLNPD LNQTKPGNFP SLAVSSNEFE PSNSHMVKSY SLLFRVTRPG RREFNGMING ETNENIDVSE ELPARRKRNR EDGEKTFVAQ MTVFDKNRRL QLLDGEYEVA MQEMEECPIS KKRATWETIL DGKRLPPFET FSQGPTLQFT LRWTGETNDK STAPVAKPLA TRNSESLHQE NKPGSVKPAQ TIAVKETLTT ELQTRKEKDN SNESRQKLRI FYQFLYNNNT RQQTEARDDL HCPWCTLNCR KLYSLLKHLK LCHSRFIFNY VYHPKGARID VSINECYDGS YAGNPQDIHR QPGFAFSRNG PVKRTPITHI LVCRPKRTKA SMSEFLESED GEVEQQRTYS SGHNRLYFHS DTCLPLRPQE MEVDSEDEKD PEWLREKTIT QIEEFSDVNE GEKEVMKLWN LHVMKHGFIA DNQMNHACML FVENYGQKII KKNLCRNFML HLVSMHDFNL ISIMSIDKAV TKLREMQQKL EKGESATPSN EEIAEEQNGT ANGFSETNSK EKALETDGVS GVPKQSKKQK L
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Top Product
    Discover our top product SUZ12 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))
    Alternative Name
    Suz12 (SUZ12 Products)
    Synonyms
    fc31h10 Protein, im:7153578 Protein, si:dkey-10p5.2 Protein, suz12 Protein, wu:fc31h10 Protein, 2610028O16Rik Protein, AI195385 Protein, AU016842 Protein, AW536442 Protein, D11Ertd530e Protein, mKIAA0160 Protein, Xsuz12 Protein, chet9 Protein, jjaz1 Protein, CHET9 Protein, ChET 9 protein Protein, JJAZ1 Protein, suz12l Protein, zgc:100863 Protein, SUZ12 polycomb repressive complex 2b subunit Protein, SUZ12 polycomb repressive complex 2 subunit Protein, suppressor of zeste 12 homolog (Drosophila) Protein, SUZ12 polycomb repressive complex 2 subunit S homeolog Protein, SUZ12 polycomb repressive complex 2a subunit Protein, suz12b Protein, Suz12 Protein, SUZ12 Protein, suz12.S Protein, suz12a Protein
    Background
    Polycomb protein Suz12 (Suppressor of zeste 12 protein homolog),FUNCTION: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. {ECO:0000250|UniProtKB:Q15022, ECO:0000269|PubMed:15385962, ECO:0000269|PubMed:15516932, ECO:0000269|PubMed:17339329}.
    Molecular Weight
    83.0 kDa
    UniProt
    Q80U70
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