ELL3 Protein (AA 1-395) (Strep Tag)

Catalog No. ABIN3136044

Product Details

Target: ELL3 (Elongation Factor RNA Polymerase II-Like 3 (ELL3))

Protein Type: Recombinant

Protein Characteristics: AA 1-395

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This ELL3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Brand: AliCE®

Sequence: MEGTQEALSG KMRLLFTPAA RTSLLMLRLN EAALRALQEC QQQQVRPVIA FQGHRGYLRF PGPGWSCLFS FIVSQCGQEG TNGGLDLVYQ RLGRSGPNCL HCLGSLRERL TIWAAMDTIP APLLAQEHLT EGTRESESWQ DTGDEPEGHP QLAPDEVSDP LASHHEQSLP GSSSEPMAQW EMRNHTYLPS REPDQSLLSP ASQKRLDKKR SAPITTEEPE EKRLRALPLA SSPLQGLANQ DSQEGEDWGQ DEDEEGDEDG DSRLEQSLSA PSASESPSPE EVPDYLLQYR AIHSTEQQQA YEQDFETDYA EYRILHARVG AASQRFTELG AEIKRLQRGT PEHKVLEDKI VQEYKKFRKR YPSYREEKHR CEYLHQKLSH IKGLILEFEE KNRGS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ELL3 (Elongation Factor RNA Polymerase II-Like 3 (ELL3))

Alternative Name: Ell3 (ELL3 Products)

Background: RNA polymerase II elongation factor ELL3,FUNCTION: Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT). {ECO:0000269|PubMed:22768269, ECO:0000269|PubMed:23273992}.

Molecular Weight: 44.8 kDa

UniProt: Q80VR2

Pathways: Negative Regulation of intrinsic apoptotic Signaling