Claspin Protein (CLSPN) (AA 1-1315) (Strep Tag)

Catalog No. ABIN3136084

Product Details

Target: Claspin (CLSPN)

Protein Type: Recombinant

Protein Characteristics: AA 1-1315

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This Claspin protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Brand: AliCE®

Sequence: MTGEVGSEVN LEVNDLKLLS QEAADSPVDS GQGSFETLEP LSERDSDEEI FVSKKPKSRK VLQDSDSEAE DRDDAPEKPT YDDSAEDTQE NLHSGKSQSR SFPKALADSD ESDMEETPSQ ESPETQEAPS LEPGHQTGHS VDFTTGRKLS KTLLREGAEG KAKSKRRLEK EERTMEKIRR LKKKETRCEE SDADRPLNDS GCLLEDSDLF ETGLEEENDS ALEDEESLES IRAAVKNKVK NRKKKEPTLE SEAFSLEDGN ELSKGSARKE RKAARLSKEA LKKLHSETQR LVRESALNLP YHMPESKTIH DFFKRKPRPT CQGSAMALLK SCKYQSGHYK ETVNPADAAG MGAEDSSRGS EQRTGAGIAA ETNVLSEVSE EAGITAGSDE ACGKDPVRRG ELEIEETEKH SDDRPYSPGD RSMSQQESSI PRIEDNEGHQ AGDLTESDPP ALEGEELKTV EKTDAKEGMP EQKTQSAAAA AVAVVTAAAA PPEKVRRFTV DRLRQLGVDV SSQPRLGADE DSFVILDEPK TNRELEALKQ RFWRHANPAA SPRACQTVNV NIIVKDLGTN GKEELKAEVV PVTLAAEKLE GASHAKPGEK LQMLKAKLQE AMKLRRLEER QKRQALFKLD NEDGFEEEEE EEEMTDESEE DGEEETTEYL LGSEDTETKD EKETDKENTD TSSDIGKSVA LCVPKPLSSD STLLLFKDSS SKMGYFPTEE KSETDEYLAK QSDKLDEDDS SSLLTKESSH NSSFELIGST IPSYQPCNRQ IGRGASFLPT AGFRSPSPGL FRGSLISSAS KSSGKLSEPS LPVEDSQDLY TASPEPKTLF LGAGDFQFCL EDDTQSQLLD ADGFLNIRNH RHRYQAVKPQ LPLASMDENA MDANMDELLD LCTGQFTSQP EEKCQPRKND KKENMEELLN LCSGKFPTQD ASPVAPLGLR SQEKESSTED PMEEALALCS GSFPTDREEE GEEEEFGDFQ LVSKENGFAS DEDEHSDSND EELALDLEDD EEELLKQSEK MKRQMRLKKY LEDEAEVSGS DVGSEDEYDG EEIDEYEEDV IDEVLPSDEE LESQIKKIHM KTMLDDDKRR LRLYQERYLA DGDLHSDGPG RTRKFRWKHI DDTSQMDLFH RDSDDDQVEE QLDETEAKWR KERIEREQWL REQAQQGKIA ADEEDIGDDS QFMMLAKKVT AKALQKNASH TVVVQESKSV LRNPFETIRP GGAHQLKTGS LLNQPKAVLQ KLAALSDLNP SAPRNSRNFV FHTLSPTKAE AAKDSSKPQV RRRGLSSMMS PSPKRLKTNG SSPGPKRSIF RYLES
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Claspin (CLSPN)

Alternative Name: Clspn (CLSPN Products)

Background: Claspin,FUNCTION: Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation (PubMed:27401717). Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins (By similarity). Also required to maintain normal rates of replication fork progression during unperturbed DNA replication (PubMed:27401717). Binds directly to DNA, with particular affinity for branched or forked molecules and interacts with multiple protein components of the replisome such as the MCM2-7 complex and TIMELESS. Important for initiation of DNA replication, recruits kinase CDC7 to phosphorylate MCM2-7 components (By similarity). {ECO:0000250|UniProtKB:Q9HAW4, ECO:0000269|PubMed:27401717}.

Molecular Weight: 146.7 kDa

UniProt: Q80YR7