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IREB2 Protein (AA 1-963) (His tag)

Crystallography grade IREB2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3136108
$24,004.89
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Will be delivered in 70 Business Days
  • Target See all IREB2 Proteins
    IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-963
    Origin
    • 4
    • 1
    Mouse
    Source
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This IREB2 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MDSPSAGYTF EYLIETLNGN SQKKFFNVPK LGGTKYDILP YSIRVLLEAA VRNCDGFLMK KEDVMNILDW KTKQSNVEVP FFPARVVLQD FTGIPAMVDF AAMREAVKTL GGDPKKVHPA CPTDLTVDHS LQIDFSKCAI QNAPNPGGGD LQKAGKLSPL KVQSKKLPCR GQTTCRGSCD SGELSRNSGT FSSQIENTPV LCPFHLQPVP EPETVLKNQE VEFGRNRERL QFFKWSSGAF KNVAVIPPGT GMAHQVNLEY LSRVVFEETD LLFPDSVVGT DSHITMVNGL GILGWGVGGI ETEAVMLGLP VTLTLPEVVG CELTGSSNAF VTSIDIVLGI TKHLRQVGVA GKFVEFFGSG VSQLSIVDRT TIANMCPEYG AILSFFPVDN VTLRHLEHTG FDKTKLESME KYLKAVKLFR NDENSSEPEY SQVIQINLNS IVASVSGPKR PQDRVAVTDM KSDFQACLNE KVGFKGFQVA AEKQSDTVSV RYDGSEYKLS HGSVVIAAVI SCTNNCNPSV MLAAGLLAKK AVEIGLRVKP YIRTSLSPGS GMVTHYLSSS GVLPYLSKLG FDIVGYGCST CVGNTAPLSE AVLNAVKQGD LVTCGVLSGN KHFEGRLCDC VRANYLASPP LVVAYAIAGT VNIDFQTEPL GTDSTGKEIY LHDIWPSREE VHQMEEEHVI LSMFKTLKEK VEMGNKRWNS LEAPDSVLFP WDVKSTYIRC PSFFDKLTKE PAASQPIENA HVLLYLGDSV TTDHISPAGS IARSSAAAKY LTNRGLTPRE FNSYGARRGN DAVMTRGTFA NIKLFNKFIG KPAPKTIHFP SGQTLDVFEA AELYQKEGIP LIILAGKKYG SGNSRDWAAK GPYLLGVKAV LAESYEKIHK DHLIGIGIAP LEFLPGENAD SLGLSGREVF SLSFPEELFP GITLNIKTST GKEFSVIASF ANDVEITLYK HGGLLNFVAR KFL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ireb2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))
    Alternative Name
    Ireb2 (IREB2 Products)
    Synonyms
    IREB2 Protein, im:7153062 Protein, DKFZp468N047 Protein, D9Ertd85e Protein, Irp2 Protein, ACO3 Protein, IRP2 Protein, IRP2AD Protein, IREBP2 Protein, iron responsive element binding protein 2 Protein, iron-responsive element binding protein 2 Protein, iron responsive element binding protein 2 L homeolog Protein, IREB2 Protein, ireb2 Protein, Ireb2 Protein, ireb2.L Protein
    Background
    RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA (By similarity). {ECO:0000250}.
    Molecular Weight
    105.9 kDa Including tag.
    UniProt
    Q811J3
    Pathways
    Transition Metal Ion Homeostasis
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