HIP1 Protein (AA 1-1029) (Strep Tag)

Catalog No. ABIN3136715

Product Details

Target: HIP1 (Huntingtin Interacting Protein 1 (HIP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1029

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This HIP1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MDRMASSMKQ VSNPLPKVLS RRGVGAGMEA AERESFERTQ TVSVNKAINT QEVAVKEKHA RTCILGTHHE KGAQTFWSVV NRLPLSSNAM LCWKFCHVFH KLLRDGHPNV LKDSLRYKNE LSDMSRMWGH LSEGYGQLCS IYLKLLRTRM EYHTKNPRFP GNLQMSDRQL DEAGESDVNN FFQLTVEMFD YLECELNLFQ TVFNSLDMSR SVSVTTAGQC RLAPLIQVIL DCSHLYDYTV KLLFKLHSCL PADTLQGHRD RFMEQFTKLK DLFQRSSNLQ YFKRLIQIPQ LPENPPNFLR ASALSEHISP VVVIPAEVSS PDSEPVLEKD DLMDMDASQQ TLFDNKFDDV FGSSLSSDPF NFNNQNGVNK DEKDHLIERL YREISGLTGQ LDNMKIESQR AMLQLKGRVS ELEAELAEQQ HLGRQAMDDC EFLRTELDEL KRQREDTEKA QRSLTEIERK AQANEQRYSK LKEKYSELVQ NHADLLRKNA EVTKQVSVAR QAQVDLEREK KELADSFART QEQQDVLENL KHELATSRQE LQVLHSNLET SAQSEAKWLT QIAELEKEQG SLATVAAQRE EELSALRDQL ESTQIKLAGA QESMCQQVKD QRKTLLAGIR KAAEREIQEA LSQLEEPTLI SCAGSTDHLL SKVSSVSSCL EQLEKNGSQY LACPEDISEL LHSITLLAHL TGDTIIQGSA TSLRAPPEPA DSLTEACRQY GRETLAYLSS LEEEGTMENA DVTALRNCLS RVKTLGEELL PRGLDIKQEE LGDLVDKEMA ATSAAIEAAT TRIEEILSKS RAGDTGVKLE VNERILGSCT SLMQAIKVLV VASKDLQKEI VESGRGTASP KEFYAKNSRW TEGLISASKA VGWGATIMVD AADLVVQGKG KFEELMVCSR EIAASTAQLV AASKVKANKG SLNLTQLQQA SRGVNQATAA VVASTISGKS QIEETDSMDF SSMTLTQIKR QEMDSQVRVL ELENDLQKER QKLGELRKKH YELAGVAEGW EEGTEASPST VQEAIPDKE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HIP1 (Huntingtin Interacting Protein 1 (HIP1))

Alternative Name: Hip1 (HIP1 Products)

Synonyms: HIP-I Protein, ILWEQ Protein, CG10971 Protein, Dmel\\CG10971 Protein, Hip1R Protein, 2610109B09Rik Protein, A930014B11Rik Protein, E130315I21Rik Protein, mKIAA4113 Protein, huntingtin interacting protein 1 Protein, Huntingtin interacting protein 1 Protein, HIP1 Protein, Hip1 Protein, hip1 Protein

Background: Huntingtin-interacting protein 1 (HIP-1) (Huntingtin-interacting protein I) (HIP-I),FUNCTION: Plays a role in clathrin-mediated endocytosis and trafficking (PubMed:11577110). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (PubMed:12839988, PubMed:17329427). Regulates presynaptic nerve terminal activity (PubMed:17928447). Enhances androgen receptor (AR)-mediated transcription (By similarity). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (By similarity). Binds 3-phosphoinositides (via ENTH domain) (By similarity). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (By similarity). May play a functional role in the cell filament networks (By similarity). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed:11604514, PubMed:14998932, PubMed:16967501, PubMed:17928447). {ECO:0000250|UniProtKB:O00291, ECO:0000269|PubMed:11577110, ECO:0000269|PubMed:11604514, ECO:0000269|PubMed:12839988, ECO:0000269|PubMed:14998932, ECO:0000269|PubMed:16967501, ECO:0000269|PubMed:17329427}.

Molecular Weight: 115.2 kDa

UniProt: Q8VD75

Pathways: Positive Regulation of Endopeptidase Activity