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GPR31 Protein (AA 1-319) (rho-1D4 tag)

Crystallography grade GPR31 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3131197
$18,625.61
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
Delivery in 50 to 55 Business Days
  • Target See all GPR31 Proteins
    GPR31 (G Protein-Coupled Receptor 31 (GPR31))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-319
    Origin
    Mouse
    Source
    • 1
    Insect Cells
    Purification tag / Conjugate
    This GPR31 protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MERTNCSAAS TVVETAVGTM LTLECVLGLM GNAVALWTFF YRLKVWKPYA VYLFNLVVAD LLLATSLPFF AAFYLKGKTW KLGHMPCQVL LFLLAFSRGV GVAFLTTVAL DRYLRVVHPR LRVNLLSLRA AWGISSLIWL LMVVLTPQNL LTCRTTQNST ECPSFYPTGG AKAIATCQEV LFFLQVLLPF GLISFCNSGL IRTLQKRLRE SDKQPRIRRA RVLVAIVLLL FGLCFLPSVL TRVLVHIFQE FKSCSVQQAI VRASDIAGSL TCLHSTLSPA IYCFSNPAFT HSYRKVLKSL RGRRKAAESP SDNLRDSYS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Gpr31 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    GPR31 (G Protein-Coupled Receptor 31 (GPR31))
    Alternative Name
    Gpr31 (GPR31 Products)
    Synonyms
    12-HETER Protein, HETER Protein, HETER1 Protein, GPR31 Protein, G protein-coupled receptor 31 Protein, GPR31 Protein, Gpr31 Protein
    Background
    High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B (By similarity). {ECO:0000250}.
    Molecular Weight
    36.8 kDa Including tag.
    UniProt
    F8VQN3
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