SLC26A1 Protein (AA 1-704) (rho-1D4 tag)

Catalog No. ABIN3120590

Product Details

Target: SLC26A1 (Solute Carrier Family 26 (Sulfate Transporter), Member 1 (SLC26A1))

Protein Type: Recombinant

Protein Characteristics: AA 1-704

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This SLC26A1 protein is labelled with rho-1D4 tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: MDASPEPQQK GGTLVLVRRQ PPVSQGLLET LKARLKKSCT CSMPCAQALV QGLFPAIHWL PQYRLKEYLA GDVMSGLVIG IILVPQAIAY SLLAGLQPIY SLYTSFFANL IYFLMGTSRH VNVGIFSLLC LMVGQVVDRE LQLAGFDPSQ DSLGPKNNDS TLNNSATTLI IGLQDCRRDC YAIRVATALT LMAGLYQVLM GILRLGFVST YLSQPLLDGF AMGASVTILT SQAKHMLGVQ IPRHQGLGMV VHTWLSLLQN VGQANICDVV TSALCLGVLL AAKELSDRYR HRLKVPIPTE LFVIVVATIV SHFGQLHTRF DSRVAGNIPT GFVAPQVPDP KIMWRVALDA MSLALVGSAF SISLAEMFAR SHGYSVSANQ ELLAVGCCNV LPAFFHCFAT SAALSKTLVK IATGCQTQLS SVVSAAVVLL VLLVLAPLFH DLQRCVLACI IVVSLRGALR KVKDLPQLWR LSPADALVWV ATAATCVLVS TEAGLLAGVF FSLLSLAGRT QRPRAALLAR IGDSTFYEDA AEFEGLLPPP EVRVFRFTGP LYYANKDFFL RSLYRLTGLD AGHSATRKDQ GPEVGVSNRS LVDGKDLGSV SSGAGLVVPL AFGFHTVVID CAPLLFLDVA GMATLKDLRR DYRALDITLL LACCSPSVRD TLRKGGFLGE EQGAENELLF PSVHSAVEAA CARREELLAA DSAL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Slc26a1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC26A1 (Solute Carrier Family 26 (Sulfate Transporter), Member 1 (SLC26A1))

Alternative Name: Slc26a1 (SLC26A1 Products)

Synonyms: edm4 Protein, sat1 Protein, sat-1 Protein, MGC86347 Protein, SLC26A1 Protein, sb:cb565 Protein, wu:fe23c03 Protein, zgc:158435 Protein, EDM4 Protein, SAT-1 Protein, SAT1 Protein, Sat1 Protein, solute carrier family 26 (anion exchanger), member 1 L homeolog Protein, solute carrier family 26 member 1 Protein, solute carrier family 26 (anion exchanger), member 1 Protein, solute carrier family 26 (sulfate transporter), member 1 Protein, slc26a1.L Protein, SLC26A1 Protein, slc26a1 Protein, Slc26a1 Protein

Background: High affinity uptake of sulfate. Accepts oxalate, but not succinate as a cosubstrate. Mediates sulfate and oxalate transport. {ECO:0000269|PubMed:12217875}.

Molecular Weight: 77.0 kDa Including tag.

UniProt: P58735

Pathways: Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process, Dicarboxylic Acid Transport