O3FAR1 Protein (AA 1-361) (Strep Tag)

Catalog No. ABIN3135912

Product Details

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Protein Type: Recombinant

Protein Characteristics: AA 1-361

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This O3FAR1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MSPECAQTTG PGPSHTLDQV NRTHFPFFSD VKGDHRLVLS VVETTVLGLI FVVSLLGNVC ALVLVARRRR RGATASLVLN LFCADLLFTS AIPLVLVVRW TEAWLLGPVV CHLLFYVMTM SGSVTILTLA AVSLERMVCI VRLRRGLSGP GRRTQAALLA FIWGYSALAA LPLCILFRVV PQRLPGGDQE IPICTLDWPN RIGEISWDVF FVTLNFLVPG LVIVISYSKI LQITKASRKR LTLSLAYSES HQIRVSQQDY RLFRTLFLLM VSFFIMWSPI IITILLILIQ NFRQDLVIWP SLFFWVVAFT FANSALNPIL YNMSLFRNEW RKIFCCFFFP EKGAIFTDTS VRRNDLSVIS S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: O3FAR1 (omega-3 Fatty Acid Receptor 1 (O3FAR1))

Alternative Name: Ffar4 (O3FAR1 Products)

Synonyms: BMIQ10 Protein, GPR120 Protein, GPR129 Protein, GT01 Protein, O3FAR1 Protein, PGR4 Protein, AI552415 Protein, Gpr120 Protein, Gpr129 Protein, KPG_013 Protein, O3far1 Protein, Pgr4 Protein, free fatty acid receptor 4 Protein, phosphodiesterase 6C Protein, FFAR4 Protein, Ffar4 Protein, PDE6C Protein

Background: Free fatty acid receptor 4 (G-protein coupled receptor 120) (G-protein coupled receptor GT01) (Omega-3 fatty acid receptor 1),FUNCTION: G-protein-coupled receptor for long-chain fatty acids (LCFAs) with a major role in adipogenesis, energy metabolism and inflammation. Signals via G-protein and beta-arrestin pathways (PubMed:27852822, PubMed:26873857). LCFAs sensing initiates activation of phosphoinositidase C-linked G proteins GNAQ and GNA11 (G(q)/G(11)), inducing a variety of cellular responses via second messenger pathways such as intracellular calcium mobilization, modulation of cyclic adenosine monophosphate (cAMP) production, and mitogen-activated protein kinases (MAPKs) (PubMed:27852822, PubMed:26873857). After LCFAs binding, associates with beta-arrestin ARRB2 that acts as an adapter protein coupling the receptor to specific downstream signaling pathways, as well as mediating receptor endocytosis (PubMed:27852822, PubMed:26873857). In response to dietary fats, plays an important role in the regulation of adipocyte proliferation and differentiation (PubMed:17250804, PubMed:22343897, PubMed:27853148, PubMed:29343498, PubMed:31761534). Acts as a receptor for omega-3 polyunsaturated fatty acids (PUFAs) at primary cilium of perivascular preadipocytes, initiating an adipogenic program via cAMP and CTCF-dependent chromatin remodeling that ultimately results in transcriptional activation of adipogenic genes and cell cycle entry (PubMed:31761534). Induces differentiation of brown and beige adipocytes probably via autocrine and endocrine functions of FGF21 hormone (PubMed:27853148, PubMed:29343498). Contributes to the thermogenic activation of brown adipose tissue and the browning of white adipose tissue (PubMed:27853148, PubMed:29343498). Activates brown adipocytes by initiating intracellular calcium signaling leading to mitochondrial depolarization and fission, and overall increased mitochondrial respiration (PubMed:29343498). Consequently stimulates fatty acid uptake and oxidation in mitochondria together with UCP1-mediated thermogenic respiration, eventually reducing fat mass (PubMed:29343498). Regulates bi-potential differentiation of bone marrow mesenchymal stem cells toward osteoblasts or adipocytes likely by up-regulating distinct integrins (PubMed:26365922). In response to dietary fats regulates hormone secretion and appetite (PubMed:15619630, PubMed:25535828, PubMed:24742677, PubMed:24663807, PubMed:24222669). Stimulates GIP and GLP1 secretion from enteroendocrine cells as well as GCG secretion in pancreatic alpha cells, thereby playing a role in the regulation of blood glucose levels (PubMed:15619630, PubMed:25535828, PubMed:24742677). Negatively regulates glucose-induced SST secretion in pancreatic delta cells (PubMed:24663807). Mediates LCFAs inhibition of GHRL secretion, an appetite-controlling hormone (PubMed:24222669). In taste buds, contributes to sensing of dietary fatty acids by the gustatory system (PubMed:20573884). During the inflammatory response, promotes anti-inflammatory M2 macrophage differentiation in adipose tissue (PubMed:20813258). Mediates the anti-inflammatory effects of omega-3 PUFAs via inhibition of NLRP3 inflammasome activation (By similarity). In this pathway, interacts with adapter protein ARRB2 and inhibits the priming step triggered by Toll-like receptors (TLRs) at the level of TAK1 and TAB1 (PubMed:20813258). Further inhibits the activation step when ARRB2 directly associates with NLRP3, leading to inhibition of pro-inflammatory cytokine release (By similarity). Mediates LCFAs anti-apoptotic effects (PubMed:15774482). {ECO:0000250|UniProtKB:Q5NUL3, ECO:0000269|PubMed:15619630, ECO:0000269|PubMed:15774482, ECO:0000269|PubMed:17250804, ECO:0000269|PubMed:20573884, ECO:0000269|PubMed:20813258, ECO:0000269|PubMed:22343897, ECO:0000269|PubMed:24222669, ECO:0000269|PubMed:24663807, ECO:0000269|PubMed:24742677, ECO:0000269|PubMed:25535828, ECO:0000269|PubMed:26365922, ECO:0000269|PubMed:26873857, ECO:0000269|PubMed:27852822, ECO:0000269|PubMed:27853148, ECO:0000269|PubMed:29343498, ECO:0000269|PubMed:31761534}.

Molecular Weight: 40.8 kDa

UniProt: Q7TMA4

Pathways: Hormone Transport