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WNT3A Protein (AA 19-352)

WNT3A Origin: Mouse Host: Mammalian Cells Recombinant 70-75 % Func Active
Catalog No. ABIN4948749
  • Target See all WNT3A Proteins
    WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 19-352
    Origin
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 8
    • 5
    • 2
    • 1
    • 1
    • 1
    Mammalian Cells
    Application
    Functional Studies (Func)
    Specificity
    Stem cell or organoid culture
    Purification
    This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
    Purity
    70-75 %
    Endotoxin Level
    Less than 0.1 ng/μg (1 IEU/μg)
    Biological Activity Comment
    Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.
  • Application Notes
    31-60 ng/mL
    To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
    Handling Advice
    Mix the protein by pipetting up and down but not by vortexing
    Storage
    -20 °C
    Storage Comment
    Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
  • Target
    WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
    Alternative Name
    Wnt3a (WNT3A Products)
    Synonyms
    WNT3A Protein, Wnt-3a Protein, vt Protein, Zwnt[a] Protein, wnt3 Protein, wnt3 l Protein, wnt3l Protein, wnt[a] Protein, Xwnt-3a Protein, wnt-3a Protein, wnt3a-A Protein, xwnt3a Protein, WNT-3A Protein, WNT3 Protein, wingless-type MMTV integration site family, member 3A Protein, Wnt family member 3A Protein, wingless-type MMTV integration site family member 3A L homeolog Protein, WNT3A Protein, Wnt3a Protein, wnt3a Protein, wnt3a.L Protein
    Background
    Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene
    Molecular Weight
    41 kDa evaluated by SDS-PAGE under reducing conditions
    Gene ID
    22416
    NCBI Accession
    NC_000077
    UniProt
    P27467
    Pathways
    WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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