Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) (Active) Protein

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Protein Name
  • WNT3A
  • Wnt-3a
  • vt
  • Zwnt[a]
  • wnt3
  • wnt3 l
  • wnt3l
  • wnt[a]
  • Xwnt-3a
  • wnt-3a
  • wnt3a-A
  • xwnt3a
  • WNT-3A
  • WNT3
  • wingless-type MMTV integration site family, member 3A
  • Wnt family member 3A
  • wingless-type MMTV integration site family member 3A L homeolog
  • WNT3A
  • Wnt3a
  • wnt3a
  • wnt3a.L
Protein Characteristics
AA 19-352
14
3
1
1
1
1
1
Origin
Mouse (Murine)
13
6
1
1
1
1
1
1
1
Source
Mammalian Cells
11
7
6
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func)
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Specificity Stem cell or organoid culture
Purification This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
Purity 70-75 %
Endotoxin Level Less than 0.1 ng/μg (1 IEU/μg)
Biological Activity Comment Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.
Protein Name
Background Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene
Molecular Weight 41 kDa evaluated by SDS-PAGE under reducing conditions
Gene ID 22416
NCBI Accession NC_000077
UniProt P27467
Pathways WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
Application Notes 31-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only
Restrictions For Research Use only
Format Liquid
Buffer Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
Handling Advice Mix the protein by pipetting up and down but not by vortexing
Storage -20 °C
Storage Comment Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
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