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HIST1H3J Protein (His tag)

HIST1H3J Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN5505657
  • Target
    HIST1H3J (Histone Cluster 1, H3j (HIST1H3J))
    Protein Type
    Recombinant
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This HIST1H3J protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human Purified recombinant protein of Human histone cluster 1, H3j (HIST1H3J), full length, with N-terminal HIS tag, expressed in E.coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
    • Produced with end-sequenced ORF clone
    Purification
    Purified
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the N-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    HIST1H3J (Histone Cluster 1, H3j (HIST1H3J))
    Alternative Name
    histone cluster 1, H3j
    Synonyms
    H3/j Protein, H3FJ Protein, histone cluster 1 H3 family member j Protein, HIST1H3J Protein
    Background
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
    Molecular Weight
    15.2 kDa
    NCBI Accession
    NP_003526
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