ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4) protein (His tag)

Details for Product No. ABIN5505775
Protein Name
Origin
Human
3
2
1
1
Source
Escherichia coli (E. coli)
4
2
1
Protein Type
Recombinant
Purification tag / Conjugate
His tag
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human Purified recombinant protein of Human ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4), transcript variant 2 , full length, with N-terminal HIS tag, expressed in E.coli, 50 μg (full length, N-term HIS tag, transcript variant 2) protein expressed in E.coli.
  • Produced with end-sequenced ORF clone
Purification Purified
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells. [UniProtKB/Swiss-Prot Function]
Molecular Weight 19.1 kDa
NCBI Accession NP_778222
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the N-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Image no. 1 for ST8 alpha-N-Acetyl-Neuraminide alpha-2,8-Sialyltransferase 4 (ST8SIA4) protein (His tag) (ABIN5505775) Validation with Western Blot