VISTA/B7-H5 (AA 33-191) protein (Fc Tag)
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- Target
- VISTA/B7-H5
- Protein Type
- Recombinant
- Protein Characteristics
- AA 33-191
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- Fc Tag
- Application
- SDS-PAGE (SDS)
- Purpose
- VISTA [B7-H5] (mouse):Fc (human) (rec.)
- Cross-Reactivity
- Mouse
- Characteristics
- The extracellular domain of mouse VISTA (aa 33-191) is fused at the C-terminus to the Fc portion of human IgG1.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 1 mg/mL
- Buffer
- Contains PBS.
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- VISTA/B7-H5
- Alternative Name
- VISTA [B7-H5]
- Background
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V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation, Platelet Receptor Gi24, Stress-induced Secreted Protein-1, Sisp-1, V-set Domain-containing Immunoregulatory Receptor
Mouse VISTA is a 55- to 65 kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells.
- Molecular Weight
- ~65-70kDa (SDS-PAGE)
- UniProt
- Q9D659
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