OspC Protein
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- Target
- OspC
- Protein Type
- Recombinant
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Origin
- Borrelia burgdorferi
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Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB), Control Peptide (CP), SDS-PAGE (SDS)
- Supplier Product No.
- 000-001-c11
- Supplier
- Rockland
- Purpose
- OspC Control Protein
- Purification
- OspC is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspC and was estimated to be greater than 90% pure.
- Sterility
- Sterile filtered
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- Application Notes
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Application Note: OspC is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 63.1 kDa for OspC-MBP, (20.7 kDa for OspC and 42.4 for MBP) in size corresponding to OspC by Western blotting in the appropriate cell lysate or extract. Outer Surface Protein C has been tested in SDS-page and western blot.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: Lateral Flow Assay: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
-
- Target
- OspC
- Background
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Synonyms: OspC, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, control protein
Background: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
- Gene ID
- 1194415
- NCBI Accession
- WP_010890595
- UniProt
- Q07337
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