CAPSD (AA 228-781) protein (His tag)

Catalog No. ABIN5711083

Product Details

Target: CAPSD

Protein Type: Recombinant

Protein Characteristics: AA 228-781

Origin: Human Parvovirus B19 (HPV B19)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Sequence: MTSVNSAEAS TGAGGGGSNS VKSMWSEGAT FSANSVTCTF SRQFLIPYDP EHHYKVFSPA ASSCHNASGK EAKVCTISPI MGYSTPWRYL DFNALNLFFS PLEFQHLIEN YGSIAPDALT VTISEIAVKD VTDKTGGGVQ VTDSTTGRLC MLVDHEYKYP YVLGQGQDTL APELPIWVYF PPQYAYLTVG DVNTQGISGD SKKLASEESA FYVLEHSSFQ LLGTGGTASM SYKFPPVPPE NLEGCSQHFY EMYNPLYGSR LGVPDTLGGD PKFRSLTHED HAIQPQNFMP GPLVNSVSTK EGDSSNTGAG KALTGLSTGT SQNTRISLRP GPVSQPYHHW DTDKYVTGIN AISHGQTTYG NAEDKEYQQG VGRFPNEKEQ LKQLQGLNMH TYFPNKGTQQ YTDQIERPLM VGSVWNRRAL HYESQLWSKI PNLDDSFKTQ FAALGGWGLH QPPPQIFLKI LPQSGPIGGI KSMGITTLVQ YAVGIMTVTM TFKLGPRKAT GRWNPQPGVY PPHAAGHLPY VLYDPTATDA KQHHRHGYEK PEELWTAKSR VHPL

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: CAPSD

Target Type: Viral Protein

Background: Capsid protein self-assbles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangents leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal mbrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids .

Molecular Weight: 64.89 kDa

UniProt: P07299