VP2 (AA 2-351) protein (His tag)

Catalog No. ABIN5714200

Product Details

Target: VP2

Protein Type: Recombinant

Protein Characteristics: AA 2-351

Origin: BK Polyomavirus (BKPyV)

Source: Yeast

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Sequence: GAALALLGDL VASVSEAAAA TGFSVAEIAA GEAAAAIEVQ IASLATVEGI TSTSEAIAAI GLTPQTYAVI AGAPGAIAGF AALIQTVSGI SSLAQVGYRF FSDWDHKVST VGLYQQSGMA LELFNPDEYY DILFPGVNTF VNNIQYLDPR HWGPSLFATI SQALWHVIRD DIPSITSQEL QRRTERFFRD SLARFLEETT WTIVNAPINF YNYIQQYYSD LSPIRPSMVR QVAEREGTRV HFGHTYSIDD ADSIEEVTQR MDLRNQQSVH SGEFIEKTIA PGGANQRTAP QWMLPLLLGL YGTVTPALEA YEDGPNQKKR RVSRGSSQKA KGTRASAKTT NKRRSRSSRS

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: VP2

Target Type: Viral Protein

Background: Isoform VP2 is a structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Participates in host cell receptor binding together with VP1. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum mbrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum mbrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or Vp3 nuclear localization signal (shared C-terminus). Plays a role in virion assbly within the nucleus in particular through a DNA-binding domain located in the C-terminal region. A N-terminal myristoylation suggests a scaffold function for virion assbly .Isoform VP3: structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum mbrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum mbrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or Vp3 nuclear localization signal (shared C-terminus). Isoform VP3 plays a role in virion assbly within the nucleus. May participate in host cell lysis when associated with VP4 .Isoform VP4 is a viroporin inducing perforation of cellular mbranes to trigger virus progeny release. Forms pores of 3 nm inner diameter. VP4 is expressed about 24 hours after the late structural proteins and is not incorporated into the mature virion .

Molecular Weight: 40.2 kDa

UniProt: P03094