Parkin Protein (AA 1-465) (His tag)
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- Target See all Parkin (PARK2) Proteins
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-465
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Parkin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MIVFVRFNSS HGFPVEVDSD TSIFQLKEVV AKRQGVPADQ LRVIFAGKEL RNDWTVQNCD LDQQSIVHIV QRPWRKGQEM NATGGDDPRN AAGGCEREPQ SLTRVDLSSS VLPGDSVGLA VILHTDSRKD SPPAGSPAGR SIYNSFYVYC KGPCQRVQPG KLRVQCSTCR QATLTLTQGP SCWDDVLIPN RMSGECQSPH CPGTSAEFFF KCGAHPTSDK ETSVALHLIA TNSRNITCIT CTDVRSPVLV FQCNSRHVIC LDCFHLYCVT RLNDRQFVHD PQLGYSLPCV AGCPNSLIKE LHHFRILGEE QYNRYQQYGA EECVLQMGGV LCPRPGCGAG LLPEPDQRKV TCEGGNGLGC GFAFCRECKE AYHEGECSAV FEASGTTTQA YRVDERAAEQ ARWEAASKET IKKTTKPCPR CHVPVEKNGG CMHMKCPQPQ CRLEWCWNCG CEWNRVCMGD HWFDV
- Purity
- > 80 % by SDS - PAGE
- Top Product
- Discover our top product PARK2 Protein
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Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-465) protein (His tag)
custom-made PARK2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-165) protein (His-IF2DI Tag)PARK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 288-387) protein (GST tag)PARK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-387) protein (GST tag)PARK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-464) protein (His tag)custom-made PARK2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
- Alternative Name
- PARK2 (PARK2 Products)
- Background
- The precise function of this gene is unknown, however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. Recombinant human PARK2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 53.8 kDa (485aa)
- NCBI Accession
- NP_004553
- UniProt
- O60260
- Pathways
- Autophagy, Ubiquitin Proteasome Pathway
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