EEF2 Protein (AA 574-858) (His tag)

Catalog No. ABIN5853814

Product Details

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Protein Type: Recombinant

Protein Characteristics: AA 574-858

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EEF2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSDPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL

Purity: > 90 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol, 0.4M urea

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2 (EEF2 Products)

Background: EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molecular Weight: 34.3 kDa (308aa)

NCBI Accession: NP_001952

UniProt: P13639

Pathways: AMPK Signaling