SRSF1 Protein (AA 1-248) (His tag)

Catalog No. ABIN5854856

Product Details

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-248

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SRSF1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH

Purity: > 95 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 100  mM KCl, 1  mM DTT, 0.2  mM EDTA, 40 % Glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Alternative Name: SRSF1 (SRSF1 Products)

Background: SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation. Recombinant human SRSF1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 28.5kDa (254aa) 28-40KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_008855

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization