Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag)

Details for Product No. ABIN6239762
Protein Name
Protein Characteristics
AA 1203-1332
4
3
3
2
1
1
1
1
1
1
1
1
1
1
Origin
Human
46
19
2
1
1
1
1
Source
Escherichia coli (E. coli)
61
2
1
1
Biological Activity
Active
Purification tag / Conjugate
This IL16 protein is labelled with His tag.
Application
Activity Assay (AcA), Cell Culture (CC)
Options
Characteristics Tag location: N-terminal His Tag
Purity > 97 %
Biological Activity Comment Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleaved into different isoforms. Researches have shown that the recombinant human IL16, containing C-terminal 130 amino acids, has same bioactivity as the natural secreted human IL16. Besides, IL16 has been considered to stimulate the proliferation of Jurkat cells at low dose (10-9 M). Thus, a proliferation assay of recombinant human IL16 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL16. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL16 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL16 significantly promoted cell proliferation of Jurkat cells.
Protein Name
Background Alternative Names: LCF, PrIL-16, Lymphocyte Chemoattractant Factor, Pro-Interleukin 16
Molecular Weight 17kDa
UniProt Q14005
Application Notes Isoelectric Point: 6
Restrictions For Research Use only
Format Lyophilized
Buffer 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Preservative Dithiothreitol (DTT), Other preservative, ProClin
Precaution of Use This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Supplier Images
Image no. 1 for Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (ABIN6239762) Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleave...
Image no. 2 for Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (ABIN6239762) Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (Image 2)
Image no. 3 for Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (ABIN6239762) Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) (Image 3)