GSTP1 Protein (AA 1-210) (His tag)
GSTP1
Origin: Human
Host: Escherichia coli (E. coli)
> 95 %
AcA, CC
Active
1 image
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- Target See all GSTP1 Proteins
- GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))
- Biological Activity
- Active
- Protein Characteristics
- AA 1-210
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GSTP1 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GSTp with CDK5.
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- Discover our top product GSTP1 Protein
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Glutathione S-Transferase pi 1 (GSTP1) (AA 1-210) protein (GST tag)
GSTP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Isoelectric Point: 6.2
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))
- Alternative Name
- Glutathione S Transferase Pi (GSTp) (GSTP1 Products)
- Background
- Alternative Names: GSTP1, GST-P1, DFN7, FAEES3, GST3
- Molecular Weight
- 25kDa
- UniProt
- P09211
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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