CXCL12 Protein (AA 23-89) (His tag,GST tag)

Catalog No. ABIN6239854

Product Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Biological Activity: Active

Protein Characteristics: AA 23-89

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CXCL12 protein is labelled with His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His and GST Tag

Purity: > 97 %

Biological Activity Comment: SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and SDF1 (10ng/mL, 30ng/mL and 60ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows SDF1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of SDF1 occurs at 10ng/mL.

Application Details

Application Notes: Isoelectric Point: 8.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: Stromal Cell Derived Factor 1 (SDF1) (CXCL12 Products)

Background: Alternative Names: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12

Molecular Weight: 40kDa

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling