Glutathione Peroxidase 2/GPX2 (U40C) (AA 1-190) protein (His tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95% by SDS-PAGE
SDS
1 image
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- Target
- Glutathione Peroxidase 2/GPX2 (U40C)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-190
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAFIAKSFYD LSAISLDGEK VDFNTFRGRA VLIENVASLC GTTTRDFTQL NELQCRFPRR LVVLGFPCNQ FGHQENCQNE EILNSLKYVR PGGGYQPTFT LVQKCEVNGQ NEHPVFAYLK DKLPYPYDDP FSLMTDPKLI IWSPVRRSDV AWNFEKFLIG PEGEPFRRYS RTFPTINIEP DIKRLLKVAI
- Characteristics
- GPX2(U40C), 1-190aa, Human, His tag, E.coli
- Purity
- > 95% by SDS-PAGE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH7.5) containing 40% glycerol, 0.15M NaCl, 1mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- Glutathione Peroxidase 2/GPX2 (U40C)
- Background
- GPX2, also known as glutathione peroxidase 2, belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. GPX2 could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
- Molecular Weight
- 24.1 kDa (210aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_002074
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