DESI1 Protein (AA 1-168) (His tag)
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- Target See all DESI1 (PPPDE2) Proteins
- DESI1 (PPPDE2) (PPPDE Peptidase Domain Containing 2 (PPPDE2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-168
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DESI1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMEPPNLY PVKLYVYDLS KGLARRLSPI MLGKQLEGIW HTSIVVHKDE FFFGSGGISS CPPGGTLLGP PDSVVDVGST EVTEEIFLEY LSSLGESLFR GEAYNLFEHN CNTFSNEVAQ FLTGRKIPSY ITDLPSEVLS TPFGQALRPL LDSIQIQPPG GSSVGRPNGQ S
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product PPPDE2 Protein
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PPPDE Peptidase Domain Containing 2 (PPPDE2) (AA 1-168) protein (GST tag)
PPPDE2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
PPPDE Peptidase Domain Containing 2 (PPPDE2) (AA 1-168) protein (Strep Tag)Crystallography grade PPPDE2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
PPPDE Peptidase Domain Containing 2 (PPPDE2) (AA 1-168) protein (Strep Tag)Crystallography grade PPPDE2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
PPPDE Peptidase Domain Containing 2 (PPPDE2) protein (Myc-DYKDDDDK Tag)PPPDE2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DESI1 (PPPDE2) (PPPDE Peptidase Domain Containing 2 (PPPDE2))
- Alternative Name
- DESI1 (PPPDE2 Products)
- Synonyms
- D15Wsu75e Protein, DESI2 Protein, DJ347H13.4 Protein, DeSI-1 Protein, FAM152B Protein, PPPDE2 Protein, AI427858 Protein, AI850401 Protein, Fam152b Protein, Pppde2 Protein, RGD1305776 Protein, fam152b Protein, pppde2 Protein, desumoylating isopeptidase 1 Protein, desumoylating isopeptidase 1 L homeolog Protein, DESI1 Protein, Desi1 Protein, desi1.L Protein
- Background
- DESI1 belongs to the DeSI family and contains 1 PPPDE peptidase domain. This protein is a protease which deconjugates SuMO1, SuMO2 and SuMO3 from some substrate proteins and has isopeptidase but not SuMO-processing activity. DESI1 desumoylates ZBTB46. Recombinant human DESI1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 20.7 kDa (191aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_056519
- UniProt
- Q6ICB0
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