NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) (Active) protein (His tag)

Details for Product No. ABIN6387717
Protein Name
Protein Characteristics
AA 22-169
3
2
1
1
Origin
Human
6
1
1
Source
Escherichia coli (E. coli)
5
2
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
His tag
Application
SDS-PAGE (SDS)
Options
Sequence MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
Purity > 95 % by SDS - PAGE
Biological Activity Comment Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Protein Name
Background NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Molecular Weight 19.1 kDa (169aa), confirmed by MALDI-TOF
NCBI Accession NP_002504
UniProt Q13232
Pathways Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Bioactivity Validated

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 50 % glycerol, 0.1M NaCl, 2  mM DTT
Storage 4 °C,-20 °C,-80 °C
Storage Comment Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Supplier Images
Image no. 1 for NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) (Active) protein (His tag) (ABIN6387717) NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) (Active) protein (His tag)