CST3 Protein (AA 21-140) (His tag)
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- Target See all CST3 Proteins
- CST3 (Cystatin C (CST3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 21-140
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CST3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ATPKQGPRML GAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNL TDCPFHDQPH LMRKALCSFQ IYSVPWKGTH SLTKFSCKNA HHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C
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- Discover our top product CST3 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CST3 (Cystatin C (CST3))
- Alternative Name
- Cystatin-C (CST3 Products)
- Background
- CST3, also known as Cystatin-C is a member of family 2 of the cystatin superfamily. This protein is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy. Recombinant Mouse CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 14.2kDa (126aa) confirmed by MALDI-TOF 13.5-18KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_034106
- UniProt
- P21460
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