ENO1 Protein (AA 1-434) (His tag)
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- Target See all ENO1 Proteins
- ENO1 (Enolase 1 (ENO1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-434
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ENO1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.
- Top Product
- Discover our top product ENO1 Protein
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Enolase 1 (ENO1) (AA 1-434) protein (GST tag)
ENO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Enolase 1 (ENO1) protein (His tag)ENO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Enolase 1 (ENO1) (AA 1-434) proteinENO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Enolase 1 (ENO1) (AA 325-434) protein (GST tag)ENO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Enolase 1 (ENO1) (AA 1-434) (Active) proteinENO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS Active
Enolase 1 (ENO1) (AA 2-433) protein (His tag)ENO1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Enolase 1 (ENO1) (AA 2-428), (partial) protein (GST tag)ENO1 Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
Enolase 1 (ENO1) (AA 2-433), (partial) protein (His tag)ENO1 Origin: Mouse Host: Yeast Recombinant > 90 % ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.5) containing 40 % glycerol, 0.1M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ENO1 (Enolase 1 (ENO1))
- Alternative Name
- Eno1 (ENO1 Products)
- Background
- Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 49.5 kDa (457aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_075608
- UniProt
- P17182
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