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Hba-x Protein (AA 1-142) (His tag)

HBA-X Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
Catalog No. ABIN6388171
  • Target
    Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-142
    Origin
    Human
    Source
    Baculovirus infected Insect Cells
    Purification tag / Conjugate
    This Hba-x protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Sequence
    MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH
    Purity
    > 85 % by SDS - PAGE
    Endotoxin Level
    < 1.0 EU per 1ug of protein (determined by LAL method)
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    Liquid. In 50  mM Tris-HCl (  pH 7.5) containing 30 % glycerol, 0.5M NaCl, 2  mM DTT, 0.1  mM PMSF
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))
    Alternative Name
    Hba-x
    Synonyms
    AI450015 Protein, Hbz Protein, hemoglobin X, alpha-like embryonic chain in Hba complex Protein, Hba-x Protein
    Background
    RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molecular Weight
    60.2kDa (533aa) 70-100kDa (SDS-PAGE under reducing conditions.)
    NCBI Accession
    NP_003152
    UniProt
    P23443
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