NTH (AA 1-211) protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 85 % by SDS - PAGE
SDS
1 image
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- Target
- NTH
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-211
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Characteristics
- nth, 1-211aa, E.coli, His tag, E.coli
- Purity
- > 85 % by SDS - PAGE
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
- Storage
- 4 °C
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- Target
- NTH
- Background
- Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase. NCBI no.: NP_416150
- Molecular Weight
- 25.7 kDa (231aa) confirmed by MALDI-TOF
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