DDIT4 Protein (AA 1-232) (His tag)

Catalog No. ABIN667871

Product Details

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Protein Type: Recombinant

Protein Characteristics: AA 1-232

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DDIT4 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: DDIT4, 1-232aa, Human, His tag, E.coli

Purity: > 90 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Storage: 4 °C

Target Details

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: DDIT4 (DDIT4 Products)

Background: DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: DNA damage-inducible transcript 4 protein, Dig2, FLJ20500, REDD1, RP11-442H21.1, RTP801. NCBI no.: NP_061931

Molecular Weight: 27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process