MYLPF Protein (AA 1-169) (His tag)
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- Target See all MYLPF Proteins
- MYLPF (Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-169
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MYLPF protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- MYLPF, 1-169aa, Human, His tag, E.coli
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product MYLPF Protein
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Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF) (AA 1-169) protein (His tag)
MYLPF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF) (AA 1-169) protein (GST tag)MYLPF Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF) (AA 2-169) protein (His-SUMO Tag)MYLPF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF) protein (Myc-DYKDDDDK Tag)MYLPF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
- Storage
- 4 °C
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- Target
- MYLPF (Myosin Light Chain, phosphorylatable, Fast Skeletal Muscle (MYLPF))
- Alternative Name
- MYLPF (MYLPF Products)
- Background
- Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development. Recombinant human MYLPF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Myosin regulatory light chain 2, skeletal muscle isoform, MRLC2, MYL11, Fast skeletal myosin light chain 2, MLC2B. NCBI no.: NP_037424
- Molecular Weight
- 21.2kDa (189aa), confirmed by MALDI-TOF
- Pathways
- Myometrial Relaxation and Contraction
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