BCKDK Protein (GST tag)

Catalog No. ABIN6699557

Supplier Product No.: 009-001-r48s

Product Details

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This BCKDK protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r48s

Supplier: Rockland

Purpose: BCKDK recombinant protein-GST fusion protein

Purification: Recombinant human BCKDK (30-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Application Note: BCKDK Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. BCKDK Protein is suitable for use in Western Blot. Expect a band approximately ~ 69 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: BCKDK Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Alternative Name: BCKDK (BCKDK Products)

Background:

Synonyms: [BCKDKD, BDK, 3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDHKIN

Background: BCKDK or branched chain alpha-keto acid dehydrogenase kinase is an important regulator of the valine, leucine, and isoleucine catabolic pathways. BCKDK is found in the mitochondrion where it phosphorylates and inactivates BCKD. The branched-chain alpha-keto dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the oxidation of branched-chain amino acids and BCKDK inactivates the BCKDH complex by catalyzing serine phosphorylation of the E1-alpha subunit (1). BCKDH kinase catalyzes the serine phosphorylation of the E1- alpha subunit of BCKDH (2). BCKDK Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, and Metabolic Disorder research.

NCBI Accession: NM_005881

Pathways: SARS-CoV-2 Protein Interactome