BUB1 Protein (GST tag)
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- Target See all BUB1 Proteins
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This BUB1 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r54s
- Supplier
- Rockland
- Purpose
- BUB1 recombinant protein-GST fusion protein
- Purification
- Recombinant human BUB1 (756-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
- Purity
- >80%
- Top Product
- Discover our top product BUB1 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: BUB1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 63 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1 μg/μL
- Buffer
- BUB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
- Alternative Name
- BUB1 (BUB1 Products)
- Background
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Synonyms: BUB1A, BUB1L, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1
Background: BUB1 is a kinase which involved in spindle checkpoint function that in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. BUB1 is highly expressed in thymus, ovary, and testis (1). BUB1 maintains the steady-state level and centromeric localization of SGO1, and they concluded that BUB1 protects centromeric cohesion in mitosis through SGO1 (2). BUB1-mediated phosphorylation of CDC20 is required for proper checkpoint signaling and that inhibition of APC by BUB1 may partly account for the sensitivity of the spindle checkpoint. BUB1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes. BUB1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.
- NCBI Accession
- NM_004336
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