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BUB1 Protein (GST tag)

BUB1 Origin: Human Host: Insect cells (Sf9) Recombinant >80% WB
Rockland
Catalog No. ABIN6699561
Supplier Product No.: 009-001-r54s
  • Target See all BUB1 Proteins
    BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
    Protein Type
    Recombinant
    Origin
    • 6
    • 2
    Human
    Source
    • 3
    • 2
    • 2
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This BUB1 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r54s
    Supplier
    Rockland
    Purpose
    BUB1 recombinant protein-GST fusion protein
    Purification
    Recombinant human BUB1 (756-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
    Purity
    >80%
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    Discover our top product BUB1 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: BUB1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 63 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    BUB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
    Alternative Name
    BUB1 (BUB1 Products)
    Background

    Synonyms: BUB1A, BUB1L, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1

    Background: BUB1 is a kinase which involved in spindle checkpoint function that in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. BUB1 is highly expressed in thymus, ovary, and testis (1). BUB1 maintains the steady-state level and centromeric localization of SGO1, and they concluded that BUB1 protects centromeric cohesion in mitosis through SGO1 (2). BUB1-mediated phosphorylation of CDC20 is required for proper checkpoint signaling and that inhibition of APC by BUB1 may partly account for the sensitivity of the spindle checkpoint. BUB1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes. BUB1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

    NCBI Accession
    NM_004336
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