SNAP91 Protein (His tag)

Catalog No. ABIN6699569

Supplier Product No.: 009-001-r55s

Product Details

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SNAP91 protein is labelled with His tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r55s

Supplier: Rockland

Purpose: Calmodulin recombinant protein-HIS Epitope

Purification: Recombinant full-length human Calmodulin1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: Calmodulin 1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 17 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Calmodulin 1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Alternative Name: CALM (SNAP91 Products)

Background:

Synonyms: CALM1, CALML2, CAMI, DD132, PHKD, Calmodulin, CaM, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CAM3, CAMC, CAMIII

Background: Calmodulin 1 is a member of calcium-modulated proteins which is present in the cytosol and on membranes facing the cytosol and has a high affinity for calcium. Calmodulin 1 has 4 calcium-binding domains and plays a role in cell growth, cell cycle, signal transduction and the synthesis and release of neurotransmitters. Calmodulin can bind to the epidermal growth factor receptor at its cytosolic juxtamembrane region and this inhibits its tyrosine kinase activity (1). A number of other proteins including HSP70 have been shown to interact with Calmodulin 1 in a cell-phase-specific manner (2). Calmodulin 1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, and PKA/PKC Pathway research.

NCBI Accession: NM_006888