CREB1 Protein (GST tag)

Catalog No. ABIN6699573

Supplier Product No.: 012-001-r73s

Product Details

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CREB1 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 012-001-r73s

Supplier: Rockland

Purpose: CREB1 recombinant protein-GST fusion protein

Purification: Recombinant rat CREB1 (1-280) was expressed in E.coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Purity: >70%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: CREB1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 59 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: CREB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: Creb1 (CREB1 Products)

Background:

Synonyms: cAMP Responsive Element Binding Protein 1, creb, Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1

Background: CREB1 is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in response to extracellular signals and are essential regulators of adaptive behavior and long-term memory formation (1). Activation of CREB1 by protein kinase A-mediated phosphorylation has been implicated in the survival of mammalian cells. Mice lacking CREB in the central nervous system during development show extensive apoptosis of postmitotic neurons (2). CREB binds as homo- and heterodimers to promoters containing CRE and activator protein 1 (AP-1) sites to alter target-gene expression). CREB1 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, and PKA/PKC Pathway research.

NCBI Accession: NM_031017

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation