CTNNA1 Protein (GST tag)

Catalog No. ABIN6699575

Supplier Product No.: 009-001-r57s

Product Details

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This CTNNA1 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r57s

Supplier: Rockland

Purpose: Catenin alpha recombinant protein recombinant protein-GST fusion protein

Purification: Recombinant full-length human Catenin α was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: Catenin α Protein is suitable for use in Western Blot. Expect a band approximately ~ 123 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Catenin α Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: CTNNA1 (CTNNA1 Products)

Background:

Synonyms: CTNNA1, CAP102, FLJ36832,Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13

Background: Catenin α is a novel actin-binding and -bundling protein. Catenin α is responsible for organizing and tethering actin filaments at the zones of E-cadherin-mediated cell-cell contact (1). Monomeric Catenin α can bind strongly to E-Cadherin-β-Catenin, whereas the dimer preferentially binds actin filaments. Different molecular conformations are associated with these different binding states, indicating that Catenin α is an allosteric protein. Catenin α directly regulates actin-filament organization by suppressing Arp2/3-mediated actin polymerization, likely by competing with the Arp2/3 complex for binding to actin filaments (2). Catenin α Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Cancer, Cardiovascular Disease, Invasion/Metastasis, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.

NCBI Accession: NM_001903

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location