CTNNB1 Protein (GST tag)
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- Target See all CTNNB1 Proteins
- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This CTNNB1 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r58s
- Supplier
- Rockland
- Purpose
- Catenin beta recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human Catenin β was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
- Purity
- >80%
- Top Product
- Discover our top product CTNNB1 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: Catenin β Protein is suitable for use in Western Blot. Expect a band approximately ~ 115 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- Catenin β Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
- Alternative Name
- CTNNB1 (CTNNB1 Products)
- Background
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Synonyms: CTNNB, FLJ25606, Catenin beta-1, Beta-catenin
Background: β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins associate with E-cadherin at cellular junctions (1). β-catenin interacts with TCF and LEF transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3β, form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of β-catenin by the proteasome. APC and Siah-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas (2). Catenin β Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Cancer, Cardiovascular Disease, Invasion/Metastasis, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.
- NCBI Accession
- NM_001904
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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