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CDC34 Protein (His tag)

CDC34 Origin: Human Host: Escherichia coli (E. coli) Recombinant >75% WB
Rockland
Catalog No. ABIN6699591
Supplier Product No.: 009-001-u42s
  • Target See all CDC34 Proteins
    CDC34 (Cell Division Cycle 34 (CDC34))
    Protein Type
    Recombinant
    Origin
    Human
    Source
    • 4
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This CDC34 protein is labelled with His tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-u42s
    Supplier
    Rockland
    Purpose
    UBE2R1 recombinant protein-HIS Epitope
    Purification
    Recombinant full length human UBE2R1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >75% by densitometry.
    Purity
    >75%
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    Discover our top product CDC34 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: UBE2R1 Protein is suitable for use in Western Blot. Expect a band approximately ~33 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    UBE2R1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    CDC34 (Cell Division Cycle 34 (CDC34))
    Alternative Name
    CDC34 (CDC34 Products)
    Background

    Synonyms: E2-CDC34, UBC3, UBCH3, CDC34, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34

    Background: UBE2R1 is a member of the ubiquitin-conjugating enzyme family which catalyzes the covalent attachment of ubiquitin to other proteins. UBE2R1 is a part of the large multiprotein complex which is required for ubiquitin-mediated degradation of cell cycle G1 regulators and for the initiation of DNA replication (1). CK2 can phosphorylate CDC34 and UBC3B at corresponding serine residue in the C terminus of each protein. Phosphorylated UBC3B and CDC34 binds specifically to the F-box protein beta-TRCP which results in enhanced degradation of beta-catenin, a substrate of BTRC. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition (2). UBE2R1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

    NCBI Accession
    NM_004359
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