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CDC7 Protein (GST tag)

CDC7 Origin: Human Host: Insect cells (Sf9) Recombinant >70% WB
Rockland
Catalog No. ABIN6699595
Supplier Product No.: 009-001-r61s
  • Target See all CDC7 Proteins
    CDC7 (Cell Division Cycle 7 (CDC7))
    Protein Type
    Recombinant
    Origin
    • 3
    • 2
    Human
    Source
    • 2
    • 2
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This CDC7 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r61s
    Supplier
    Rockland
    Purpose
    CDC7 recombinant protein-GST fusion protein
    Purification
    Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
    Purity
    >70%
    Top Product
    Discover our top product CDC7 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: CDC7 Protein is suitable for use in Western Blot. Expect a band approximately ~ 94 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    CDC7 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    CDC7 (Cell Division Cycle 7 (CDC7))
    Alternative Name
    CDC7 (CDC7 Products)
    Background

    Synonyms: CDC7L1, HsCDC7, Hsk1, huCDC7, MGC117361, MGC126237, MGC126238, Cell division cycle 7-related protein kinase, CDC7-related kinase,

    Background: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the cell division cycle. Overexpression of CDC7 gene product may be associated with neoplastic transformation for some tumors. Inhibition of CDC7 in cancer cells impairs progression through S phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability (1). Inhibition of CDC7 kinase activity in cancer cells restricts DNA replication and induces apoptosis. CDC7 phosphorylates the minichromosome maintenance protein 2 (Mcm2), a component of the DNA replicative helicase needed for genome duplication (2). CDC7 Protein is ideal for investigators involved in Signaling Proteins, Cell-Cycle Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

    NCBI Accession
    NM_003503
    Pathways
    Mitotic G1-G1/S Phases, DNA Replication
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