CDC7 Protein (GST tag)
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- Target See all CDC7 Proteins
- CDC7 (Cell Division Cycle 7 (CDC7))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This CDC7 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r61s
- Supplier
- Rockland
- Purpose
- CDC7 recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human CDC7 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
- Purity
- >70%
- Top Product
- Discover our top product CDC7 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: CDC7 Protein is suitable for use in Western Blot. Expect a band approximately ~ 94 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- CDC7 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- CDC7 (Cell Division Cycle 7 (CDC7))
- Alternative Name
- CDC7 (CDC7 Products)
- Background
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Synonyms: CDC7L1, HsCDC7, Hsk1, huCDC7, MGC117361, MGC126237, MGC126238, Cell division cycle 7-related protein kinase, CDC7-related kinase,
Background: CDC7 is a cell division cycle protein that is critical for the G1/S transition and initiation of DNA replication during the cell division cycle. Overexpression of CDC7 gene product may be associated with neoplastic transformation for some tumors. Inhibition of CDC7 in cancer cells impairs progression through S phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability (1). Inhibition of CDC7 kinase activity in cancer cells restricts DNA replication and induces apoptosis. CDC7 phosphorylates the minichromosome maintenance protein 2 (Mcm2), a component of the DNA replicative helicase needed for genome duplication (2). CDC7 Protein is ideal for investigators involved in Signaling Proteins, Cell-Cycle Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.
- NCBI Accession
- NM_003503
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication
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