CDK14 Protein (GST tag)

Catalog No. ABIN6699755

Supplier Product No.: 009-001-s90s

Product Details

Target: CDK14 (Cyclin-Dependent Kinase 14 (CDK14))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This CDK14 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s90s

Supplier: Rockland

Purpose: PFTK1 (CDK14) recombinant protein-GST fusion protein

Purification: Recombinant full-length human PFTK1 (CDK14) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: PFTK1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~72 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: PFTK1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CDK14 (Cyclin-Dependent Kinase 14 (CDK14))

Alternative Name: CDK14 (CDK14 Products)

Background:

Synonyms: CDK14, PFTAIRE1, Cyclin-dependent kinase 14

Background: PFTK1, also known as PFTAIRE protein kinase 1, is a member of the CDC2 -related protein kinase family which is expressed primarily in the postnatal and adult nervous system (1). PFTK1 is highly expressed in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 interacts with 14-3-3-beta, 14-3-3-epsilon, 14-3-3-eta and 14-3-3-tau. Using PFTK1 mutant constructs and in vitro and in vivo binding studies, it was shown that PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms. (2). Significant upregulation of PFTK1 expression is observed in esophageal squamous cell carcinoma (ESCC). PFTK1 is not only useful as a prognostic marker in ESCC, but also as a predictor of the response to chemotherapy. PFTK1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

NCBI Accession: NM_012395