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CDKN3 Protein (GST tag)

CDKN3 Origin: Human Host: Insect cells (Sf9) Recombinant >90% WB
Rockland
Catalog No. ABIN6699771
Supplier Product No.: 009-001-r70s
  • Target See all CDKN3 Proteins
    CDKN3 (Cyclin-Dependent Kinase Inhibitor 3 (CDKN3))
    Protein Type
    Recombinant
    Origin
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 4
    • 3
    • 2
    • 2
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This CDKN3 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r70s
    Supplier
    Rockland
    Purpose
    CDKN3 recombinant protein-GST fusion protein
    Purification
    Recombinant full-length human CDKN3 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    Purity
    >90%
    Top Product
    Discover our top product CDKN3 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: CDKN3 Protein is suitable for use in Western Blot. Expect a band approximately ~ 51 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    CDKN3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    CDKN3 (Cyclin-Dependent Kinase Inhibitor 3 (CDKN3))
    Alternative Name
    CDKN3 (CDKN3 Products)
    Background

    Synonyms: CDI1, CIP2, FLJ25787, KAP, KAP1, MGC70625, Cyclin-dependent kinase inhibitor 3, CDK2-associated dual-specificity phosphatase, Cyclin-dependent kinase interactor 1 , Cyclin-dependent kinase-interacting protein 2, Kinase-associated phosphatase

    Background: CDKN3 is a member of the dual specificity protein phosphatase family that acts as a cyclin-dependent kinase inhibitor (1). CDKN3 has been shown to interact with and dephosphorylate specifically the CDK2 kinase thereby preventing it's activation. CDKN3 has been reported to be deleted, mutated, or overexpressed in several kinds of cancers. Increased expression of CDKN3 leads to increased levels of kinase-associated phosphatase activity that inhibits the G(1)/S transition of the cell cycle by dephosphorylating the cyclin-dependent kinases (2). CDKN3 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, Cell Cycle, and Ser/Thr Kinases research.

    NCBI Accession
    NM_005192
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